血管生成
一氧化氮
聚丙烯酸
纳米技术
细胞生物学
化学
细胞凋亡
细胞
内皮干细胞
血管内皮生长因子
材料科学
生物物理学
癌症研究
血管内皮生长因子受体
生物
生物化学
聚合物
体外
有机化学
作者
Hyejoong Jeong,Daheui Choi,Joo Hyun Kang,Jiwoong Heo,Jinkee Hong
标识
DOI:10.1002/adhm.202102095
摘要
The delivery of nitric oxide (NO)-an intrinsic cellular signaling molecule-is promising for disease treatment, in particular to vascular diseases, due to its endothelial-derived inherent nature. The limited diffusion distance of labile NO prompts researchers to develop various carriers and targeting methods for specific sites. In contrast to the apoptotic effect of NO, such as anticancer, delivering low NO concentration at the desired targeting area is still intricate in a physiological environment. In this study, the layer-by-layer assembled nanocoating is leveraged to develop a direct NO delivery platform to individual endothelial cells (ECs). NO can be localized to individual ECs via S-nitrosothiol-bound polyacrylic acid which is a polymer directly providing an endothelial-like constant level of NO. To increase angiogenic activation along with NO, VEGF is additionally applied to specific receptors on the cell surface. Notably, the survival and proliferation of ECs are significantly increased by a synergistic effect of NO and VEGF co-localized via nanocoating. Furthermore, the nanocoating remarkably promoted cell migration and tubule formation-prerequisites of angiogenesis. The proposed unique technology based on nanocoating demonstrates great potential for conferring desired angiogenic functions to individual ECs through efficient NO delivery.
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