HPLC‐DAD, ESI–MS/MS, and NMR of Lycopene Isolated From P. guajava L. and Its Biotechnological Applications

番茄红素 化学 阿布茨 抗氧化剂 番石榴属 电喷雾电离 高效液相色谱法 食品科学 色谱法 类胡萝卜素 质谱法 生物化学 植物 生物 DPPH
作者
Adriany G. N. Amorim,Jéssica Maria Teles Souza,Raimunda Cardoso dos Santos,Beatriz Gullón,Ana Oliveira,Luiz Fernando Arruda Santos,Adamor L. E. Virgino,Ana Carolina Mafud,Helena Maria Petrilli,Yvonne P. Mascarenhas,Cristina Delerue‐Matos,Manuela Pintado,José Roberto S. A. Leite
出处
期刊:European Journal of Lipid Science and Technology [Wiley]
卷期号:120 (3) 被引量:21
标识
DOI:10.1002/ejlt.201700330
摘要

Psidium guajava L. have been reported to be a rich source of antioxidant compounds. Its carotenoids have been highlighted by their high antioxidant capacity, which offers several benefits for human health. In this sense, lycopene isomers need to be identified. In this work, the comprehensive chemical characterization, by HPLC‐DAD, MS/MS, and NMR, of lycopene isolated from P. guajava L., antioxidant and antimicrobial activity of lycopene extracts and isolated lycopene are evaluated. The FTIR results reported a structure with Z configuration, confirmed by UV‐Vis, with λmax = 448, 473, and 505 nm for 5 ‐ Z lycopene. Furthermore, MS/MS positive ionization shows one fragment m/z 309 [M‐227] + , relatively abundant for isolated lycopene. Experimental and Theoretical NMR studies revealed that guava may contain 5‐Z lycopene because of the similarity found among the peaks. Lycopene extracts presented higher antioxidant activity than isolated lycopene, from both P. guajava L. and tomato, when measured by ABTS and ORAC ( r 2 = 0.9995 and r 2 = 0.9992, respectively). In addition, lycopene extract shows antibacterial efficacy against E. coli, S. aureus , and L. innocua , presenting MBC values of 20 mg mL −1 . These results suggest that lycopene extract have potential applicability for food, cosmetics, and pharmaceutical industry. Practical Applications : Lycopene from P. guajava L. is characterized by HPLC‐DAD, MS, NMR, FTIR, and X‐Ray, presented antioxidant capacity by ORAC and antibacterial efficacy against food pathogens. Lycopene is isolated from P. guajava L., and lycopene extracts produced are characterized by HPLC‐DAD and ESI‐MS/MS. Lycopene isolated from P. guajava L. following the process may be 5‐Z isomer of lycopene.
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