亚硫酸氢盐测序
计算生物学
生物
DNA甲基化
CpG站点
甲基化
表观遗传学
遗传学
基因
基因表达
作者
Karolina A. Åberg,Robin F. Chan,Lin Xie,Andrey A. Shabalin,Edwin J. C. G. van den Oord
出处
期刊:Methods in molecular biology
日期:2017-12-10
卷期号:: 171-189
被引量:21
标识
DOI:10.1007/978-1-4939-7481-8_10
摘要
Detailed biological knowledge about the potential importance of the methylome is typically lacking for common diseases. Therefore, methylome-wide association studies (MWAS) are critical to detect disease relevant methylation sites. Methyl-CpG-binding domain sequencing (MBD-seq) offers potential advantages compared to antibody-based enrichment, but performance depends critically on using an optimal protocol. Using an optimized protocol, MBD-seq can approximate the sensitivity/specificity obtained with whole-genome bisulfite sequencing, but at a fraction of the costs and time to complete the project. Thus, MBD-seq offers a comprehensive first pass at the CpG methylome and is economically feasible with the samples sizes required for MWAS.
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