等压标记
串联质量标签
蛋白质组
串联质谱法
定量蛋白质组学
质谱法
色谱法
蛋白质组学
化学
样品制备
磷蛋白
选择性反应监测
蛋白质质谱法
计算生物学
生物
生物化学
磷酸化
基因
作者
Hediye Erdjument‐Bromage,Fang-Ke Huang,Thomas A. Neubert
出处
期刊:Methods in molecular biology
日期:2018-01-01
卷期号:: 135-149
被引量:31
标识
DOI:10.1007/978-1-4939-7659-1_11
摘要
Quantitative proteome analysis allows comparisons of protein or phosphoprotein levels across multiple cell types or conditions. A number of experimental approaches have been described toward quantitative proteomics. In this chapter, we focus on Tandem Mass Tag (TMT) isobaric labeling of peptides for global, relative quantitation of proteins and phosphopeptides. To date, there has been no published protocol describing chemical labeling of small amounts of peptides specifically extracted from small tumor samples, for which rigorous sample preparation is necessary to ensure reproducible TMT labeling.
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