化学
荧光
溶酶体
溶血酶-
罗丹明
细胞毒性
生物物理学
光化学
生物化学
体外
探测器
酶
物理
工程类
闪烁体
电气工程
生物
量子力学
作者
Guangle Niu,Panpan Zhang,Weimin Liu,Mengqi Wang,Hongyan Zhang,Jiasheng Wu,Liping Zhang,Pengfei Wang
标识
DOI:10.1021/acs.analchem.6b04417
摘要
The development of near-infrared fluorescent probes with low pKa, high selectivity, high photostability, and high sensitivity for lysosomal pH detection is of great importance. In the present work, we developed a novel near-infrared lysosomal pH probe (Lyso-hNR) based on a rhodamine derivative. Lyso-hNR showed fast, highly sensitive, and highly selective fluorescence response to acidic pH caused by the H+-induced structure changes from the nonfluorescent spirolactam form to the highly emissive open-ring form. Lyso-hNR displays a significant fluorescence enhancement at 650 nm (over 280-fold) from pH 7.0 to 4.0 with a pKa value of 5.04. Live cell imaging data revealed that Lyso-hNR can selectively monitor lysosomal pH changes with excellent photostability and low cytotoxicity. In addition, Lyso-hNR can be successfully used in tracking lysosomal pH changes induced by chloroquine and those during apoptosis. All these features render Lyso-hNR a promising candidate to investigate lysosome-associated physiological and pathological processes.
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