医学
椎间盘
间充质干细胞
纤维蛋白胶
再生(生物学)
病理
干细胞
骨髓
解剖
外科
细胞生物学
生物
作者
Krishna Prabhu,Manish Baldia,Sunithi Mani,Noel Walter,Sanjay Kumar,Alok Srivastava
出处
期刊:Neurology India
[Medknow Publications]
日期:2021-01-01
卷期号:69 (6): 1565-1565
被引量:4
标识
DOI:10.4103/0028-3886.333531
摘要
Back pain and radicular pain due to disc degeneration are probably the most common problems encountered in neurosurgical practice. The experience and results of stem cell therapy in animal disc degeneration model will help us while doing clinical trials.To study the effect of bone marrow-derived mesenchymal stem cells in an established mouse disc degeneration model.An easily reproducible mouse coccygeal (Co) 4-5 disc degenerated model by CT-guided percutaneous needle injury was established. The mesenchymal stem cells (MSCs) were cultured from mouse bone marrow and validated. By an established technique, 24 mice disc degenerative models were generated and divided equally into 3 groups (test, placebo, and control). The test group received MSCs with fibrin glue scaffold and placebo group received only scaffold after 6 weeks of degeneration. The control group did not receive any injection. The effects of MSCs were analyzed 8 weeks post injection.The test group showed a significant change in disc height index (%) in micro CT, whereas in the placebo and control groups, there was no change. The Safranin O staining showed an increase in glycosaminoglycan content and the polarized imaging of picrosirius red staining showed restoration of the collagen fibers in annulus fibrosus, which was statistically significant.Intradiscal MSC injection restored disc height and promoted regeneration in the discs at the end of 8 weeks. MSC's niche depends on the microenvironment of the host tissue. These findings will be helpful for clinical trials.
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