TRPV4型
瞬时受体电位通道
TRPV1型
TRPM8型
TRPV公司
压电1
固有层
细胞生物学
化学
尿路上皮细胞
钙信号传导
受体
尿路上皮
膀胱
信号转导
离子通道
生物
内科学
医学
病理
生物化学
上皮
机械敏感通道
作者
Mengmeng Zhao,Zhenghao Chen,Lei Liu,Ning Ding,Jiliang Wen,Jiaxin Liu,WenZhen Wang,Nan Ge,Shulu Zu,Wei Song,Guoqing Chen,Xiulin Zhang
标识
DOI:10.3389/fphys.2021.762847
摘要
The interstitial cells in bladder lamina propria (LP-ICs) are believed to be involved in sensing/afferent signaling in bladder mucosa. Transient receptor potential (TRP) cation channels act as mechano- or chemo-sensors and may underlie some of the sensing function of bladder LP-ICs. We aimed to investigate the molecular and functional expression of TRP channels implicated in bladder sensory function and Piezo1/Piezo2 channels in cultured LP-ICs of the human bladder. Bladder tissues were obtained from patients undergoing cystectomy. LP-ICs were isolated and cultured, and used for real-time reverse transcription-quantitative polymerase chain reaction, immunocytochemistry, and calcium-imaging experiments. At the mRNA level, TRPA1, TRPV2, and Piezo1 were expressed most abundantly. Immunocytochemical staining showed protein expression of TRPA1, TRPV1, TRPV2, TRPV4, TRPM8, as well as Piezo1 and Piezo2. Calcium imaging using channel agonists/antagonists provided evidence for functional expression of TRPA1, TRPV2, TRPV4, Piezo1, but not of TRPV1 or TRPM8. Activation of these channels with their agonist resulted in release of adenosine triphosphate (ATP) from LP-ICs. Inhibition of TRPV2, TRPV4 and Piezo1 blocked the stretch induced intracellular Ca2+ increase. Whereas inhibition of TRPA1 blocked H2O2 evoked response in LP-ICs. Our results suggest LP-ICs of the bladder can perceive stretch or chemical stimuli via activation of TRPV2, TRPV4, Piezo1 and TRPA1 channels. LP-ICs may work together with urothelial cells for perception and transduction of mechanical or chemical signals in human-bladder mucosa.
科研通智能强力驱动
Strongly Powered by AbleSci AI