突变
甲基磺酸乙酯
生物
非生物胁迫
正向遗传学
遗传学
鉴定(生物学)
生物技术
突变
计算生物学
非生物成分
基因
表型
植物
古生物学
作者
Liuzhu Chen,Duan Liu,Mingfei Sun,Zhuo Yang,Hongyu Li,Keming Hu,Hong Yuan,Li Liu
标识
DOI:10.3389/fpls.2022.1052569
摘要
Ethyl methanesulfonate (EMS)-induced mutagenesis is a powerful tool to generate genetic resource for identifying untapped genes and characterizing the function of genes to understand the molecular basis of important agronomic traits. This review focuses on application of contemporary EMS mutagenesis in the field of plant development and abiotic stress tolerance research, with particular focuses on reviewing the mutation types, mutagenesis site, mutagen concentration, mutagenesis duration, the identification and characterization of mutations responsible for altered stress tolerance responses. The application of EMS mutation breeding combined with genetic engineering in the future plant breeding and fundamental research was also discussed. The collective information in this review will provide good insight on how EMS mutagenesis is efficiently applied to improve abiotic stress tolerance of crops with the utilization of Next-generation sequencing (NGS) for mutation identification.
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