Using fluorescence index (FI) of dissolved organic matter (DOM) to identify non-point source pollution: The difference in FI between soil extracts and wastewater reveals the principle

Traceability and quantification of agricultural non-point source pollution are of great significance to water pollution management in watersheds. In this study, fluorescence components and indices of dissolved organic matter (DOM) in the river, wastewater and soil extracts from different land use types were analyzed to screen indicators that can identify non-point source pollution in 15 independent small watersheds located at the southern Qinling piedmont (China). The results showed that DOM fluorescence components in soil extracts among different land uses didn't have significant differences. The values of humification index (HIX) did not vary obviously between soil extracts and wastewater, with the mean values ranging from 3.4 to 3.9. However, the average value of fluorescence index (FI) of effluent wastewater was about 2.1 and did not change significantly through treatment. The FI values of soil extracts were generally between 1.5 and 1.7. The FI values in most river waters were just between the FI values of wastewater and soil extracts. This phenomenon indicated that FI could be used as an indicator to distinguish point source and non-point source pollution. Besides, the correlation analysis showed a significant positive relationship between the non-point source pollution calculated by FI and δ15N. The relationship was different in January and July, but further confirmed the reliability of using FI to quantify non-point source pollution. This study demonstrated the feasibility of using FI to identify non-point source pollution. When combined with handheld fluorescence spectrometers and unmanned aerial vehicle-mounted fluorescence spectrometers, this method may be adopted more widely.