Sex dependent transcriptome responses of the diamondback moth, Plutella xylostella L. to cold stress

Temperature has fundamental influences on the performance and distribution of insects. While considerable attention has been devoted to extreme conditions, particularly extreme cold conditions, few studies have investigated effects of mild cold conditions on insects. We examined the transcriptomic changes in mid-fourth instar larvae of both sexes reared at 10 °C and 25 °C to investigate sex-dependent responses of Plutella xylostella to mild cold stress. There were 624 differentially expressed genes (DEGs) in females, the majority of which (n = 386) were down-regulated. In males 3239 genes were differentially expressed and the majority (n = 2341) were up-regulated. Only 280 DEGs were common to both sexes. In females, there were no DEGs encoding heat shock or cold shock proteins, but six of these DEGs were found in males. These differences suggest that females and males might adopt some different strategies to cope with cold stress and/or that they were affected by rearing under cold conditions to different degrees and in different ways. In addition, DEGs encoding antimicrobial peptides, cytochrome P450 monooxygenases, fatty acid-related enzymes, cuticle proteins, myofilament, and hormone-related proteins were found in both sexes under cold stress. The transcriptome study reveals unexpected sex-dependent thermal responses and provides new information of how an insect that does not diapause copes with low temperatures.