活性氧
材料科学
细胞内
谷胱甘肽
声动力疗法
下调和上调
纳米探针
免疫印迹
生物物理学
生物化学
化学
纳米技术
生物
纳米颗粒
酶
基因
作者
Ming Yang,Wenzhi Ren,Haijing Cui,Qiongyu Qin,Qiuye Wang,Weihao Zhu,Xiaoxia Wu,Chunshu Pan,Xiaopeng Qi,Aiguo Wu
标识
DOI:10.1021/acsami.3c03476
摘要
Amplifying the intracellular reactive oxygen species (ROS) level remains an urgent challenge for efficient sonodynamic therapy (SDT) of tumors. Herein, by loading ginsenoside Rk1 with manganese-doped hollow titania (MHT), a Rk1@MHT sonosensitizer was conceived to strengthen the outcome of tumor SDT. The results verify that manganese-doping remarkably elevates the UV-visible absorption and decreases the bandgap energy of titania from 3.2 to 3.0 eV, which improves ROS production under ultrasonic irradiation. Immunofluorescence and Western blot analysis demonstrate that ginsenoside Rk1 can block the critical protein of the glutathione synthesis pathway, glutaminase, thus enhancing intracellular ROS by eliminating the endogenous glutathione-depleted pathway of ROS. Manganese-doping confers the nanoprobe T1-weighted MRI function (r2/r1 = 1.41). Moreover, the in vivo tests confirm that Rk1@MHT-based SDT eradicates liver cancer in tumor-bearing mice via dual upregulation of intracellular ROS production. In summary, our study provides a new strategy for designing high-performance sonosensitizer to achieve noninvasive cancer treatment.
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