吞噬作用
吞噬体
细胞生物学
先天免疫系统
调理素
巨噬细胞
生物
免疫系统
受体
微生物学
化学
免疫学
生物化学
体外
作者
Sierra Soffiaturo,Christopher H. Choy,Roberto J. Botelho
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 25-39
标识
DOI:10.1007/978-1-0716-3338-0_3
摘要
Phagocytosis is carried out by cells such as macrophages of the immune system, whereby particulates like bacteria and apoptotic bodies are engulfed and sequestered within phagosomes for subsequent degradation. Hence, phagocytosis is important for infection resolution and tissue homeostasis. Aided by the innate and adaptive immune system, the activation of various phagocytic receptors triggers a cascade of downstream signaling mediators that drive actin and plasma membrane remodeling to entrap the bound particulate within the phagosome. Modulation of these molecular players can lead to distinct changes in the capacity and rates of phagocytosis. Here, we present a fluorescence microscopy-based technique to quantify phagocytosis using a macrophage-like cell line. We exemplify the technique through the phagocytosis of antibody-opsonized polystyrene beads and Escherichia coli. This method can be extended to other phagocytes and phagocytic particles.
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