核糖核酸
生物
细胞生物学
细胞
基因表达
信使核糖核酸
反义RNA
计算生物学
人口
活体细胞成像
基因
遗传学
人口学
社会学
作者
Felix Horns,Joe A. Martinez,Chengcheng Fan,Md. Intesaful Haque,Jamie Linton,Victoria Tobin,Leah A. Santat,Ailiena O. Maggiolo,Pamela J. Björkman,Carlos Lois,Michael B. Elowitz
出处
期刊:Cell
[Elsevier]
日期:2023-08-01
卷期号:186 (17): 3642-3658.e32
被引量:24
标识
DOI:10.1016/j.cell.2023.06.013
摘要
A system for programmable export of RNA molecules from living cells would enable both non-destructive monitoring of cell dynamics and engineering of cells capable of delivering executable RNA programs to other cells. We developed genetically encoded cellular RNA exporters, inspired by viruses, that efficiently package and secrete cargo RNA molecules from mammalian cells within protective nanoparticles. Exporting and sequencing RNA barcodes enabled non-destructive monitoring of cell population dynamics with clonal resolution. Further, by incorporating fusogens into the nanoparticles, we demonstrated the delivery, expression, and functional activity of exported mRNA in recipient cells. We term these systems COURIER (controlled output and uptake of RNA for interrogation, expression, and regulation). COURIER enables measurement of cell dynamics and establishes a foundation for hybrid cell and gene therapies based on cell-to-cell delivery of RNA.
科研通智能强力驱动
Strongly Powered by AbleSci AI