Three-year field study on the temporal response of soil microbial communities and functions to PFOA exposure

Contamination of per- and polyfluoroalkyl substances (PFAS) poses a significant threat to soil ecosystem health, yet there remains a lack of understanding regarding the responses of soil microbial communities to prolonged PFAS exposure in field conditions. This study involved a three-year field investigation to track changes in microbial communities and functions in soil subjected to the contamination of a primary PFAS, perfluorooctanoic acid (PFOA). Results showed that PFOA exposure altered soil bacterial and fungal communities in terms of diversity, composition, and structure. Notably, certain bacterial communities with a delayed reaction to PFOA contamination showed the most significant response after one year of exposure. Fungal communities were sensitive to PFOA in soil, exhibiting significant responses within just four months of exposure. After two years, the impact of PFOA on both bacterial and fungal communities was lessened, likely due to the long-term adaptation of microbial communities to PFOA. Moreover, PFOA exposure notably inhibited alkaline phosphatase activity and reduced certain phosphorus cycling-related functional genes after three years of exposure, suggesting potential disruptions in soil fertility. These new insights advance our understanding of the long-term effects of PFOA on soil microbial communities and functions at a field scale. PFOA in soil poses a significant threat to soil ecosystems health. Understanding how soil microbial communities temporally respond to prolonged PFOA exposure in field conditions is urgently needed. This is the first research to elucidate the temporal response of soil microbial communities (both bacteria and fungi) and functions to PFOA exposure through a three-year field lysimeter study. Findings in this study are crucial for informing future environmental policies and remediation strategies to mitigate the impacts of long-term PFOA contamination in soil ecosystems.