甲壳素
傅里叶变换红外光谱
化学
基质(水族馆)
固定化酶
琥珀酸酐
扫描电子显微镜
核化学
酶
色谱法
化学工程
材料科学
生物化学
高分子化学
生物
壳聚糖
生态学
工程类
复合材料
作者
Guoguang Wei,Chengfei Zhang,Ning Zhou,Bin Wu,Hui Li,Alei Zhang,Pingkai Ouyang,Kequan Chen
标识
DOI:10.1016/j.carbpol.2023.121322
摘要
In this study, co-immobilization of PLP and its dependent enzyme were investigated using a novel type of porous chitin bead (PCB). Crayfish shell was used to prepare PCB via dissolution of it to form beads, followed by the removal of CaCO3 and protein in-situ. Scanning electron microscopy, Fourier transform infrared spectroscopy, and Brunauer-Emmett-Teller method showed that the PCB had abundant porous structures with deacetylation degree of 33 % and the specific surface area of 35.87 m2/g. Then, the beads are used to co-immobilize pyridoxal 5-phosphate (PLP) and l-lysine decarboxylase fused with chitin-binding protein (SpLDC-ChBD). Laser scanning confocal microscopy revealed that the beads could co-immobilize PLP and SpLDC-ChBD successfully. In addition, a packed bed was also constructed using the PCB containing co-immobilized SpLDC-ChBD and PLP. The substrate conversion remained at 91.09 % after 48 h with 50 g/L l-lysine, which showed good continuous catalysis ability. This study provides a novel method for co-immobilization of enzyme and PLP, as well as develops a new application of waste crustacean shells.
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