化学
催化作用
共晶体系
三元运算
水溶液
催化效率
热稳定性
土曲霉
氢键
转氨酶
有机化学
组合化学
酶
化学工程
分子
合金
生物化学
计算机科学
工程类
程序设计语言
作者
Hongpeng Wang,Mercy Vimbai Masuku,Yachen Tao,Jiayao Yang,Yi Kuang,Changjiang Lyu,Jun Huang,Shengxiang Yang
出处
期刊:Molecules
[MDPI AG]
日期:2023-05-05
卷期号:28 (9): 3895-3895
被引量:1
标识
DOI:10.3390/molecules28093895
摘要
The efficient biosynthesis of chiral amines at an industrial scale to meet the high demand from industries that require chiral amines as precursors is challenging due to the poor stability and low catalytic efficiency of ω-transaminases (ω-TAs). Herein, this study adopted a green and efficient solvent engineering method to explore the effects of various aqueous solutions of deep eutectic solvents (DESs) as cosolvents on the catalytic efficiency and stability of ω-TA. Binary- and ternary-based DESs were used as cosolvents in enhancing the catalytic activity and stability of a ω-TA variant from Aspergillus terreus (E133A). The enzyme exhibited a higher catalytic activity in a ternary-based DES that was 2.4-fold higher than in conventional buffer. Moreover, the thermal stability was enhanced by a magnitude of 2.7, with an improvement in storage stability. Molecular docking studies illustrated that the most potent DES established strong hydrogen bond interactions with the enzyme's amino acid, which enhanced the catalytic efficiency and improved the stability of the ω-TA. Molecular docking is essential in designing DESs for a specific enzyme.
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