羟基酪醇
油尿苷
农业
酶
化学
制浆造纸工业
农业废弃物
生物技术
生物炼制
生物燃料
废物管理
有机化学
生物
工程类
抗氧化剂
多酚
生态学
作者
Gabriel García-Molina,E. A. Peters,Rosa Palmeri,Yaregal Awoke,Carlos Márquez‐Álvarez,Rosa M. Blanco
标识
DOI:10.1080/21655979.2024.2396647
摘要
Oleuropein (OP) is an appreciated compound present not only in fruits but also in leaves of olive trees, which can be transformed into hydroxytyrosol (HT), a substance with high antioxidant activity. In this work, the transformation of an agricultural residue containing OP (olive leaves or wastewater from mills) to the high added value compound HT is accomplished through different enzymatic strategies. Different enzymes were used, immobilized on various supports by diverse binding forces: beta-glucosidase encapsulated in siliceous material, esterases and lipases immobilized on hydrophobic supports (octyl-functionalized amorphous silica and periodic mesoporous organosilica), and esterase immobilized on amine-functionalized ordered mesoporous silica. All these biocatalysts were tested for oleuropein hydrolysis through two different reaction approaches: a) split of glucosidic bond catalyzed by beta-glucosidase (β-glu), followed by hydrolysis of the aglycon and further ester hydrolysis. 5 mg·mL
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