适体
微流控
微泡
纳米技术
外体
细胞外小泡
检出限
胶体金
表面增强拉曼光谱
纳米颗粒
拉曼光谱
材料科学
化学
计算机科学
细胞生物学
分子生物学
生物
色谱法
拉曼散射
小RNA
生物化学
基因
物理
光学
作者
Kwun Hei Willis Ho,Huang Lai,Ruolin Zhang,Haitian Chen,Wen Yin,Xijing Yan,Wangyu Hu,Ching Ying Katherine Lam,Yutian Gu,Jiaxiang Yan,Kunpeng Hu,Jingyu Shi,Mo Yang
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2024-09-05
卷期号:9 (9): 4860-4869
标识
DOI:10.1021/acssensors.4c01357
摘要
Exosomes, nanosized extracellular vesicles containing biomolecular cargo, are increasingly recognized as promising noninvasive biomarkers for cancer diagnosis, particularly for their role in carrying tumor-specific molecular information. Traditional methods for exosome detection face challenges such as complexity, time consumption, and the need for sophisticated equipment. This study addresses these challenges by introducing a novel droplet microfluidic platform integrated with a surface-enhanced Raman spectroscopy (SERS)-based aptasensor for the rapid and sensitive detection of HER2-positive exosomes from breast cancer cells. Our approach utilized an on-chip salt-induced gold nanoparticles (GNPs) aggregation process in the presence of HER2 aptamers and HER2-positive exosomes, enhancing the hot spot-based SERS signal amplification. This platform achieved a limit of detection of 4.5 log
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