滚动圆复制
放大器
自愈水凝胶
合理设计
DNA
底漆(化妆品)
纳米技术
材料科学
聚合酶
化学
生物系统
聚合酶链反应
生物
生物化学
高分子化学
有机化学
基因
作者
Svenja Moench,Phillip Lemke,Julia Weisser,Iliya D. Stoev,Kersten S. Rabe,Carmen M. Domínguez,Christof M. Niemeyer
标识
DOI:10.1002/chem.202401788
摘要
DNA hydrogels hold significant promise for biomedical applications and can be synthesized through enzymatic Rolling Circle Amplification (RCA). Due to the exploratory nature of this emerging field, standardized RCA protocols specifying the impact of reaction parameters are currently lacking. This study varied template sequences and reagent concentrations, evaluating RCA synthesis efficiency and hydrogel mechanical properties through quantitative PCR (qPCR) and indentation measurements, respectively. Primer concentration and stabilizing additives showed minimal impact on RCA efficiency, while changes in polymerase and nucleotide concentrations had a stronger effect. Concentration of the circular template exerted the greatest influence on RCA productivity. An exponential correlation between hydrogel viscosity and DNA amplicon concentration was observed, with nucleobase sequence significantly affecting both amplification efficiency and material properties, particularly through secondary structures. This study suggests that combining high‐throughput experimental methods with structural folding prediction offers a viable approach for systematically establishing structure‐property relationships, aiding the rational design of DNA hydrogel material systems.
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