癌胚抗原
生物传感器
适体
检出限
胶体金
荧光
化学
共轭体系
组合化学
生物物理学
分子生物学
纳米颗粒
色谱法
纳米技术
生物化学
材料科学
癌症
生物
遗传学
物理
有机化学
量子力学
聚合物
作者
Siyao Liu,Xingjie Yang,Peng Xiao,Wei Wang,Shaoqing Zhu,Jingzhi Chu,Chenguang Zhou
摘要
Abstract The sensitive detection of cancer biomarkers is crucial for early accurate diagnostics and therapy of cancer patients. Carcinoembryonic antigen (CEA) is a tumor‐associated antigen derived from colon cancer and embryonic tissues. In this study, we have developed a label‐free fluorescence biosensing platform for the quantification of CEA with the “turn‐on” signal output. This platform employs a label‐free strategy that incorporates an aptamer‐modified gold nanoparticle (Apt@AuNP) probe for the recognition of CEA, in combination with hybridization chain reaction (HCR) amplification. In the presence of target CEA, Apt@AuNPs selectively capture CEA, resulting in a reduction of subsequent complementary chains (CP) binding on Apt@AuNPs. The remaining CP, acting as the initiator sequence for HCR, triggers the HCR, leading to the formation of abundant G‐quadruplex structures. By employing Thioflavin T (ThT) for the formation of G‐quadruplex/ThT complexes, the biosensor exhibits a significant enhancement of the fluorescence signal. Under optimized conditions, the biosensor platform demonstrates a limit of detection of 0.03 nM and a linear range from 0.1 to 2.5 nM. Additionally, the specificity investigation reveals the high selectivity of this fluorescent biosensor. Finally, the performance of this method has been validated by successfully detecting CEA in real‐life samples, highlighting its potential for clinical applications.
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