角膜上皮
纤维化
再生(生物学)
肌成纤维细胞
细胞生物学
自愈水凝胶
基质
化学
基底膜
伤口愈合
上皮
角膜
癌症研究
医学
病理
生物
眼科
免疫学
免疫组织化学
有机化学
作者
J. Q. Huang,Tuoying Jiang,Jiqiao Qie,Xiaoyu Cheng,Yiyao Wang,Yang Ye,Zhuoheng Yang,Hongji Yan,Ke Yao,Haijie Han
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2024-12-18
卷期号:10 (51)
标识
DOI:10.1126/sciadv.adt1643
摘要
Corneal injury–induced fibrosis occurs because of corneal epithelial basement membrane (EBM) injury and defective regeneration. Corneal fibrosis inhibition and transparency restoration depend on reestablished EBM, where the collagen network provides structural stability and heparan sulfate binds corneal epithelium–derived cytokines to regulate homeostasis. Inspired by this, bioactive hydrogels (Hep@Gel) composed of collagen-derived gelatins and highly anionic heparin were constructed for scarless corneal repair. Hep@Gel resembled the barrier function of the EBM regarding surface-confined binding, long-time sequestration, and progressive degradation of IL-1, TGF-β, and PDGF-BB, which robustly inhibited the apoptosis and myofibroblast transition of keratocytes. Animal models of rabbits and nonhuman primates confirmed that Hep@Gel effectively limited the influx of inflammatory and fibrotic cytokines from the epithelium into the stroma to down-regulate the wound healing cascade, contributing to better vision quality with 73% reduced fibrosis. Hep@Gel offers a solution for preventing corneal injury–induced scarring and substituting for lamellar keratoplasty to remove scarring.
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