细胞生物学
效应器
跨膜蛋白
核糖核酸酶P
生物
核糖核酸
核糖核酸酶
清脆的
膜蛋白
内膜
化学
膜
生物化学
基因
线粒体
受体
作者
Arica R. VanderWal,Jung-Un Park,Bogdan Polevoda,Julia K. Nicosia,Adrian M. Molina Vargas,Elizabeth H. Kellogg,Mitchell R. O’Connell
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2023-04-27
卷期号:380 (6643): 410-415
被引量:14
标识
DOI:10.1126/science.abm1184
摘要
Type VI CRISPR-Cas systems use RNA-guided ribonuclease (RNase) Cas13 to defend bacteria against viruses, and some of these systems encode putative membrane proteins that have unclear roles in Cas13-mediated defense. We show that Csx28, of type VI-B2 systems, is a transmembrane protein that assists to slow cellular metabolism upon viral infection, increasing antiviral defense. High-resolution cryo–electron microscopy reveals that Csx28 forms an octameric pore-like structure. These Csx28 pores localize to the inner membrane in vivo. Csx28’s antiviral activity in vivo requires sequence-specific cleavage of viral messenger RNAs by Cas13b, which subsequently results in membrane depolarization, slowed metabolism, and inhibition of sustained viral infection. Our work suggests a mechanism by which Csx28 acts as a downstream, Cas13b-dependent effector protein that uses membrane perturbation as an antiviral defense strategy.
科研通智能强力驱动
Strongly Powered by AbleSci AI