亮度
碳量子点
量子点
谷胱甘肽
斑马鱼
化学
碳纤维
体内
核仁
生物物理学
纳米技术
材料科学
生物化学
细胞质
物理
光学
生物
酶
复合材料
生物技术
复合数
基因
作者
Yuwei Du,Lei Cao,Xinlu Li,Tongtong Zhu,Ruhong Yan,Wen‐Fei Dong,Li Li
出处
期刊:Analyst
[Royal Society of Chemistry]
日期:2023-01-01
卷期号:148 (10): 2375-2386
被引量:8
摘要
Carbon dots (CDs) with red fluorescence emission are highly desirable for use in bioimaging and trace- substance detection, with potential applications in biotherapy, photothermal therapy, and tumor visualization. Most CDs emit green or blue fluorescence, thus limiting their applicability. We report a novel fluorescent detection platform based on high-brightness red fluorescence emission carbon dots (R-CDs) co-doped with nitrogen and bromine, which exhibit pH and oxidized L-glutathione (GSSG) dual-responsive characteristics. The absolute quantum yield of the R-CDs was as high as 11.93%. We discovered that the R-CDs were able to detect acidic pH in live cells and zebrafish owing to protonation and deprotonation. In addition, GSSG was detected in vitro over a broad linear range (8-200 μM) using the R-CDs with excitation-independent emission. Furthermore, cell imaging and bioimaging experiments demonstrated that the R-CDs were highly cytocompatible and could be used as fluorescent probes to target lysosomes and nucleolus. These studies highlight the promising prospects of R-CDs as biosensing tools for bioimaging and trace-substance detection applications.
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