Alkaloidal compounds produced by a marine-derived fungus,Aspergillus fumigatus H1-04,and their antitumor activities

Aim To investigate the antitumor metabolites of marine-derived Aspergillus fumigatus H1-04.Methods The isolation procedure was guided by a bioassay using tsFT210 cells and various means of chromatography including TLC,VLC,LC,and semi-preparative HPLC were employed for the purification of aimed bioactive metabolites.Structures of the isolated compounds were investigated by spectroscopic methods and antitumor activities were assayed using human lung carcinoma A549,human leukemia HL60,human hepatic carcinoma cell BEL-7402,mouse leukemia P388,and mouse mammary carcinoma tsFT210 cells by MTT(A549 and HL60) or SRB(BEL-7402,P388 and tsFT210) method.Results and conclusion Seven alkaloids were isolated from the metabolites of Aspergillus fumigatus H1-04 and identified as fumiquinazoline J(11),fumiquinazoline F(2),fumiquinazoline G(3),fumiquinazoline C(4),fumiquinazoline A(5),tryptoquivaline J(6) and pseurotin A(7),respectively.Compounds 1-7 inhibited the proliferation of tsFT210 cells to a certain extent.Meanwhile,1,4,and 7 also inhibited the proliferation of P388,HL60,A-549,and BEL-7402 cells.Fumiquinazoline J(1) was found occurring in nature for the first time in the present study and its antitumor property was also reported for the first time.Compound 6 was isolated from the metabolites of marine-derived microorganisms for the first time.