Objective To explore the edible safety of breeding globefish fish. Methods The tetrodotoxin(TTX) content in the liver, muscle, blood, and gonads of puffer fish was determined by liquid chromatography. Results The liver and blood of the breeding(rubripes Fugu, Takifugu dolphin, daisy yellow Fugu, yellow fin Fugu) showed non-toxic or little toxic, and muscle showed non-toxic. Conclusion The puffer fish can be used as edible species. However, wild Takifugu dolphin has highly toxicity, so it is inedible.