Effect of virosecurinine on proliferation and cell cycle of mouse melanoma cell line B16

Aim:To investigate the effect and mechanism of virosecurinine on proliferation and cell cycle of mouse melanoma cell line B16.Methods: B16 cells were treated with virosecurinine in vitro.Cell proliferation was measured by MTT assay.Cell cycle was detected by flow cytometry.Results: Virosecurinine inhibited proliferation of B16 in a time and dose-dependent manner with an IC50 value of 63.34 μmol/L at 72 h.Virosecurinine induced apoptosis on B16 cells and decreased cells of S and G2/M stages.Conclusion:Virosecurinine can inhibit malignant proliferation of melanoma cells.The mechanism is associated with induced apoptosis and decreased cells of S and G2/M stage in cell cycle.