毛状根培养
农杆菌
老茧
枸杞
Murashige和Skoog培养基
生物
组织培养
动素
植物
园艺
植物生长
生长素
体外
转化(遗传学)
生物化学
替代医学
病理
基因
医学
作者
Zhong Hu,Yixin Wang,Yi‐Rui Wu,Li Wei
出处
期刊:China journal of Chinese materia medica
[China Journal of Chinese Materia Medica]
日期:2006-01-01
卷期号:31 (2): 106-110
被引量:5
摘要
Objective To study effects of the cycle of light and exogenous plant growth regulators on growth of normal root and hairy root of Lycium barbarum and establish a long-term system of in vitro culture of L. barbarum roots. Method Using normal roots from aseptic seedlings and hairy roots originated from Agrobacterium rhizogenes A4-mediated genetic transformation of L. barbarum, the growth of root segments was examined after 20 days of culture under different culture conditions. Result and conclusion When cultured on the MS medium with different plant growth regulators (IAA, IBA, NAA), normal root segments of L. barbarum could elongate and branch, and the addition of 1 mg x L(-1) IBA into the medium was the most suitable for long-term in vitro culture of L. barbarum root. When cultured on MS medium without hormone, light promoted the elongation and lateral root formation of type II hairy root, but not of type I hairy root. Among three tested hormones, IAA at low concentration was the best for growth of type I hairy root, while 1 mg x L(-1) IBA was the most suitable for growth of type II hairy root. With the increase of hormone level added into MS medium, callusing frequencies of type I, II hairy roots were increased, at the same time, the formation of lateral root from hairy root or callus was inhibited.
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