Dnd shRNA constructs cause altered PGC migration but fail to sterilize

生物 RNA干扰 小发夹RNA 斑马鱼 达尼奥 基因 细胞生物学 小RNA 核糖核酸 胚胎 计算生物学 遗传学
作者
Sandra Noble,Susan Boratynska,Vishal Saxena,Robert H. Devlin,Marc Ekker
出处
期刊:Aquaculture [Elsevier BV]
卷期号:512: 734305-734305
标识
DOI:10.1016/j.aquaculture.2019.734305
摘要

The release or escape of genetically modified fish from confined aquaculture facilities presents a potential risk to wild fish stocks and surrounding ecosystems. Thus, reliable biocontainment methods for aquaculture-relevant species are needed. Since physical barriers are not 100% effective, it is critical to develop reliable genetic-based methods for biocontainment. RNA interference (RNAi) could regulate gene expression and target genes that are essential for fish viability and/or reproduction. One such target gene, deadend (dnd), is required for primordial germ cell (PGC) development, migration and survival. While the use of RNAi technology has been successfully reported in C. elegans, M. musculus, and D. melanogaster, its use in zebrafish (Danio rerio) has been met with variable success. We targeted dnd using two different short-hairpin RNA (shRNA) approaches in zebrafish embryos, namely, 1) CMV-driven or a Gal4/UAS constructs with one double short-hairpin RNA stem-loop design consisting of two different target sites, and 2) CMV-driven to test two separate target sites independently flanked by the 5′ and 3′ sequences of the pri-miR-30e miRNA. The first shRNA strategy did not completely nor consistently abolish PGCs, based on vasa-expression, but low vasa expression was observed in some fish at 6 dpf. In contrast, the second shRNA strategy resulted in reduced and/or ectopic positioning of PGCs based on vasa expression at 2 and 6 dpf. However, neither strategy affected fertility in adult fish.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
星辰大海应助楠楠采纳,获得10
刚刚
刚刚
Qiao应助耍酷曼雁采纳,获得30
1秒前
阳佟半仙发布了新的文献求助10
1秒前
乐观的海雪完成签到,获得积分20
1秒前
2秒前
2秒前
miao发布了新的文献求助10
2秒前
moneymoney完成签到,获得积分10
2秒前
清新的Q完成签到,获得积分10
3秒前
3秒前
高高高完成签到,获得积分10
4秒前
4秒前
4秒前
4秒前
5秒前
xiaochuan完成签到,获得积分10
5秒前
尊敬的扬发布了新的文献求助10
5秒前
文静的翠安完成签到,获得积分10
5秒前
猫猫发布了新的文献求助10
5秒前
UU完成签到,获得积分10
5秒前
善良身影发布了新的文献求助10
5秒前
6秒前
DEREK发布了新的文献求助10
6秒前
xmz完成签到,获得积分10
6秒前
李太白云游四海完成签到,获得积分10
6秒前
Zgrey完成签到,获得积分10
6秒前
烂漫的汲完成签到,获得积分10
7秒前
jingyi完成签到,获得积分10
7秒前
石石刘发布了新的文献求助10
7秒前
yang完成签到,获得积分10
7秒前
zhaofw完成签到,获得积分10
8秒前
甜酒发布了新的文献求助10
8秒前
8秒前
wyl发布了新的文献求助10
8秒前
传奇3应助ty采纳,获得10
8秒前
8秒前
willow完成签到,获得积分10
8秒前
9秒前
10秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968934
求助须知:如何正确求助?哪些是违规求助? 3513835
关于积分的说明 11170238
捐赠科研通 3249167
什么是DOI,文献DOI怎么找? 1794650
邀请新用户注册赠送积分活动 875278
科研通“疑难数据库(出版商)”最低求助积分说明 804755