An improved simple LC–MS/MS method for the measurement of serum aripiprazole and its major metabolite

化学 色谱法 阿立哌唑 选择性反应监测 三级四极质谱仪 串联质谱法 质谱法 分析物 背景(考古学) 代谢物 定量分析(化学) 液相色谱-质谱法 分析化学(期刊)
作者
Matteo Caloro,Luana Lionetto,Ilaria Cuomo,Alessio Simonetti,Daniela Pucci,Simone De Persis,Barbara Casolla,Giorgio D. Kotzalidis,Antonio Sciarretta,Sergio De Filippis,Maurizio Simmaco,Paolo Girardi
出处
期刊:Journal of Pharmaceutical and Biomedical Analysis [Elsevier BV]
卷期号:62: 135-139 被引量:25
标识
DOI:10.1016/j.jpba.2012.01.003
摘要

Abstract Background and objectives Current liquid chromatographic tandem mass spectrometry (LC–MS/MS) methods to measure serum levels of aripiprazole (Ar) and dehydroaripiprazole (DHAr) are sensitive, but difficult to use in a hospital context. We aimed to develop a rapid LC–MS/MS method allowing reliable level measurement in the presence of co-administered drugs, withdrawing samples from 22 patients with acute agitation receiving 9.75 mg aripiprazole IM injection. Method We developed a sensitive and selective HPLC–MS/MS method to measure serum Ar and DHAr levels in a hospital laboratory, requiring minimal sample preparation and inferior sample volume compared to previous LC–MS/MS methods. Analytes were separated on a reversed-phase HPLC (run-time, 10 min). A triple quadrupole tandem mass spectrometer was used for quantitative analysis in positive mode by a multiple reaction monitoring. Samples were drawn 2, 4, 6, and 24 h post-injection. Results Calibration curves (2–1000 ng/mL for Ar and 3.5–500 ng/mL for DHAr) were linear, with mean correlation coefficient >0.9998. Within- and between-day precision and accuracy were within 10%. Mean recovery was 95.2 ± 4.5% for Ar and 97.6 ± 7.2% for DHAr. Ar and DHAr peaks were not affected by other co-administered psychotropic drugs. Conclusion Our method measured Ar and DHAr concentrations reliably, simply and rapidly without employing many reagents, as currently existing methods.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
4秒前
马士全发布了新的文献求助10
5秒前
孙成成发布了新的文献求助10
7秒前
勤恳凡双完成签到,获得积分20
7秒前
至幸发布了新的文献求助10
8秒前
慕青应助ff采纳,获得10
8秒前
科研战士发布了新的文献求助10
10秒前
12秒前
科目三应助马士全采纳,获得10
16秒前
penny发布了新的文献求助10
17秒前
Juan应助重要的秋尽采纳,获得10
18秒前
量子星尘发布了新的文献求助10
18秒前
Jasper应助科研战士采纳,获得10
18秒前
houfei发布了新的文献求助10
18秒前
24秒前
24秒前
左左发布了新的文献求助10
24秒前
25秒前
研友_VZG7GZ应助路路采纳,获得10
25秒前
26秒前
27秒前
颖火虫发布了新的文献求助10
29秒前
mm发布了新的文献求助10
30秒前
Mipe完成签到,获得积分10
31秒前
澡雪发布了新的文献求助10
31秒前
钱多多完成签到,获得积分10
32秒前
34秒前
雪白砖家完成签到 ,获得积分10
34秒前
吴大语完成签到,获得积分10
34秒前
虚幻初之发布了新的文献求助10
36秒前
36秒前
37秒前
我是老大应助99采纳,获得10
38秒前
bias完成签到,获得积分10
38秒前
40秒前
40秒前
Anthony_潇发布了新的文献求助10
40秒前
40秒前
汉堡包应助mm采纳,获得10
42秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3975516
求助须知:如何正确求助?哪些是违规求助? 3519930
关于积分的说明 11200130
捐赠科研通 3256278
什么是DOI,文献DOI怎么找? 1798183
邀请新用户注册赠送积分活动 877425
科研通“疑难数据库(出版商)”最低求助积分说明 806320