表位
过敏原
三聚体
低过敏性
化学
结晶
重组DNA
口服免疫疗法
球蛋白
芯(光纤)
免疫球蛋白E
抗原
抗体
生物化学
生物
免疫学
材料科学
过敏
复合材料
有机化学
基因
二聚体
作者
Cerrone Cabanos,Hiroyuki Urabe,Mary Rose Tandang‐Silvas,Shigeru Utsumi,Bunzo Mikami,Nobuyuki Maruyama
标识
DOI:10.1016/j.molimm.2011.08.004
摘要
Ara h 1, a 7S globulin, is one of the three major peanut allergens. We previously reported the crystallization of the core region of recombinant Ara h 1. Here, we present the crystal structure of the Ara h 1 core at a resolution of 2.43 Å. We also assayed the Ara h 1 core thermal stability and compared its final structure against other 7S globulins. The Ara h 1 core has a thermal denaturation temperature of 88.3 °C and a structure that is very similar to other 7S globulins. Previously identified linear IgE epitopes were also mapped on the three-dimensional structure. Most linear epitopes were found in the extended loop domains and the coils between the N- and C-terminal modules, while others were found in the less accessible β-sheets of the C-terminal core β-barrel domain of each monomer. Most of these epitopes become either slightly or significantly buried upon trimer formation, implying that allergen digestion in the gut is required for these epitopes to be accessible to immunoglobulins. Our findings also suggest that both intact and partially degraded allergens should be employed in future diagnostic and immunotherapeutic strategies.
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