Facilitated Substrate Channeling in a Self‐Assembled Trifunctional Enzyme Complex

Three enzymes, triosephosphate isomerase (orange in picture), aldolase (cyan), and fructose 1,6-bisphosphatase (purple), which contained dockerins (red), self-assembled into a static trifunctional enzyme complex through interaction with a mini-scaffoldin protein consisting of three different cohesins (green). The synthetic enzyme complex exhibited an enhanced reaction rate compared to the noncomplexed three-enzyme mixture at the same enzyme concentration.