生物传感器
葡萄糖氧化酶
材料科学
扫描电子显微镜
纳米团簇
检出限
电极
核化学
固定化酶
纳米技术
化学工程
高分子化学
分析化学(期刊)
化学
色谱法
有机化学
复合材料
物理化学
酶
工程类
作者
Gymama Slaughter,Joshua Sunday
出处
期刊:IEEE Sensors Journal
[Institute of Electrical and Electronics Engineers]
日期:2014-01-31
卷期号:14 (5): 1573-1576
被引量:26
标识
DOI:10.1109/jsen.2014.2298359
摘要
Glucose oxidase was immobilized using a poly (hydroxyethylmethacrylate)-based hydrogel composite material containing polyethylene glycol and cross-linked with tetraethyleneglycol diacrylate onto a ZnO-modified microdiamond biosensor. The ZnO-modified biosensor was grown by hydrothermal decomposition on array of gold patterned microdiamonds with an electroactive area of 18.8 mm 2 . The morphology of the ZnO-modified electrode was characterized by scanning electron microscope. A potential of +0.7 V versus Ag/AgCl reference electrode was applied to the biosensor. The hydrogel composite ZnO-modified microdiamond biosensor exhibited a linear dynamic range from 0.01 to 15 mM and a reproducible sensitivity of 31.4 μA/mM cm 2 . The experimental detection limit was 0.01 mM with a rapid response time of <;2 s.
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