氯菊酯
斑马鱼
生物
胚胎
幼虫
基因表达
雌激素
基因
细胞生物学
杀虫剂
遗传学
生态学
作者
Yuanxiang Jin,Rujia Chen,Liwei Sun,Wanyuan Wang,Lin Zhou,Weiping Liu,Zhengwei Fu
出处
期刊:Chemosphere
[Elsevier]
日期:2009-03-01
卷期号:74 (9): 1238-1244
被引量:42
标识
DOI:10.1016/j.chemosphere.2008.11.015
摘要
Enantioselectivity in the separation, toxicology, biodegradation and estrogenic activity of chiral pesticides has become a groundbreaking topic recently. In this study, real-time, quantitative polymerase chain reaction was adapted to investigate the induction of estrogen-responsive gene expression in embryo-larval zebrafish after 7 d of exposure to permethrin (PM) enantiomers. The PM enantiomers were completely separated by a chiral HPLC column. The in vivo study found that a 7 d exposure to 250 ng L−1 PM racemate and its enantiomers was sufficient to stimulate vtg1, esrα and cyp19b expression, while 1000 ng L−1 exposure significantly induced gene expression in a pattern similar to that of the control (50 ng L−1 E2), except for vtg2. Significant differences were detected between the enantiomers in the induction of estrogen-responsive gene expression. At the exposure level of 1000 ng L−1, the vtg1, esrα and cyp19b responses to the (−)-trans enantiomer were about 3.2-, 1.8- and 1.5-fold higher, respectively, than those in the group treated with (+)-trans enantiomer (p < 0.05). In the two cis-enantiomer treatment groups, (+)-cis increased the mRNA level of the cyp19b gene about 1.5-fold higher than the (−)-cis-enantiomer did. Of the four enantiomers, the (−)-trans enantiomer showed the greatest estrogenic activity. The results strongly indicate the occurrence of significant enantioselectivity in estrogenic activity of PM enantiomers exposed to embryo-larval zebrafish. These findings add to a growing body of evidence concerning enantioselectivity in the toxicity, endocrine-disrupting activity, and environmental biodegradation of chiral pesticides.
科研通智能强力驱动
Strongly Powered by AbleSci AI