DNA甲基化
生物
染色质
表观遗传学
甲基化
CpG站点
分子生物学
组蛋白
基因表达调控
基因表达
DNA
基因
乙酰化
遗传学
作者
Raffaela Pero,Silvia Peluso,Tiziana Angrisano,Concetta Tuccillo,Silvana Sacchetti,Simona Keller,Rossella Tomaiuolo,Carmelo B. Bruni,Francesca Lembo,Lorenzo Chiariotti
标识
DOI:10.1016/j.ijmm.2010.06.009
摘要
COX-2 expression is altered in gastrointestinal diseases. Helicobacter pylori (Hp) infection may have a critical role in COX-2 disregulation. We undertook this study to investigate possible chromatin and DNA methylation changes occurring early during COX-2 gene activation as a direct consequence of Hp–gastric cells interaction. We show that Hp infection is followed by different expression, chromatin and DNA methylation changes including: (i) biphasic activation of COX-2 gene; (ii) rapid remodulation of HDACs expression and activity, increased acetylation and release of HDAC from COX-2 promoter; (iii) transient gradual increase of H3 acetylation and H3K4 dimethylation and decrease of H3K9 dimethylation; (iv) late and long-lasting increase of H3K27 trimethylation; (v) rapid cyclical DNA methylation/demethylation events at 8 specific CpG sites (−176, −136, +25, +36, +57, +82, +198, +231) surrounding the COX-2 gene transcriptional start site. Our data indicate that specific chromatin and DNA methylation changes occur at COX-2 gene in the first phases of Hp exposure in cultured gastric cells as a primary response to host–parasite interaction.
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