化学
串联质谱法
质谱法
蛋白质质谱法
自上而下的蛋白质组学
电喷雾电离
离解(化学)
核糖核酸酶
质谱中的样品制备
三级四极质谱仪
离子
分析化学(期刊)
串联
选择性反应监测
色谱法
生物化学
有机化学
材料科学
基因
复合材料
核糖核酸
作者
Joseph A. Loo,Charles G. Edmonds,Richard Smith
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:1990-04-13
卷期号:248 (4952): 201-204
被引量:272
标识
DOI:10.1126/science.2326633
摘要
Tandem mass spectrometry has been used to obtain information related to portions of the primary sequence for an intact protein, bovine ribonuclease A. Multiply charged molecular ions, generated by electrospray ionization, were collisionally dissociated at low energies in a triple quadrupole mass spectrometer to yield singly and multiply charged fragment ions that can be assigned to the known sequence of the protein. Dissociation of the highly charged molecular ions resulted in pairs of complementary product ions. The higher order (gas-phase) protein structure affects the dissociation processes, as observed in comparisons of tandem mass spectra of the native and disulfide-reduced forms of ribonuclease A.
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