Phytochemical investigation on Fritillaria pallidiflora led to the isolations of one new steroidal alkaloid, (20R,22R,23R,25R)-3β,23-dihydroxy-N-methyl-veratram-13(17)-en-6-one (14) and fifteen known compounds: five steroidal saponins (1–5), three phenolic glucosides (6–8), three nucleoside compounds (9–11), one amino acid (12), four steroidal alkaloids (13–16). Compounds 12, 14 were isolated from F. pallidiflora for the first time. Compounds 1–6 were isolated from the genus Fritillaria for the first time and compounds 7–8 were isolated from the family Liliaceae for the first time. The chemotaxonomic significance of these compounds was summarized.