Directed co-evolution of an endoglucanase and a β-glucosidase in Escherichia coli by a novel high-throughput screening method

A novel high-throughput screening method for directed co-evolution of endoglucanase and β-glucosidase was constructed based on the synergy of the two enzymes using a bicistronic operon. To our knowledge, this is the first report of directed co-evolution of two cellulosic enzymes using an insoluble screening substrate via their synergistic reactions.