电穿孔
彗星试验
碎片(计算)
活力测定
心肌细胞
DNA断裂
触电
细胞凋亡
MTT法
化学
收缩(语法)
分子生物学
不可逆电穿孔
生物物理学
细胞生物学
DNA损伤
生物
程序性细胞死亡
DNA
生物化学
内分泌学
物理
生态学
量子力学
基因
作者
Iwona Kamińska,Małgorzata Kotulska,Anna Stecka,Jolanta Saczko,Małgorzata Drąg‐Zalesińska,Teresa Wysocka,Anna Choromańska,Nina Rembiałkowska,Rafał Nowicki,Jakub Marczak,Julita Kulbacka
出处
期刊:General Physiology and Biophysics
[AEPress, s.r.o.]
日期:2012-01-01
卷期号:31 (01): 19-25
被引量:69
摘要
Application of a high electric field causes an electric shock to the heart. This is utilized in defibrillation to reestablish normal contraction rhythms during dangerous arrhythmias or in cardiac arrest. If shock-induced transmembrane potentials are large enough, they can cause tissue destruction due to irreversible electroporation (EP). Also electrochemotherapy of nearby tissues may have an adverse effect on the heart. Herein, we present experimental data on effects of electroporation in culture of cardiac cells (H9C2). The electric field was applied in short pulses of 25-3250 V/cm, 50 µs each. The viability of cells was tested by MTT assay after 24 hours. For detection of DNA fragmentation, associated with apoptosis, alkaline and neutral comet assays were performed after EP. Additionally phase contrast images of cells obtained directly after EP were analyzed. Although cell images indicated disruption of cell membranes after EP with high intensities, only a few percent of apoptotic cells and no necrotic effects in the cell nucleus could be observed in comet assay tests performed 2 hours post EP. MTT viability test showed that pulse intensities above 375 V/cm are destructive for myocytes viability.
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