化学
转氨酶
过程开发
反应条件
溶剂
比例(比率)
过程(计算)
组合化学
催化作用
色谱法
酶
有机化学
工艺工程
计算机科学
工程类
物理
操作系统
量子力学
作者
Matthew D. Truppo,Nicholas J. Turner
摘要
A micro-scale technique has been developed for the process development of transaminase catalysed reactions. This pH indicator based, colorimetric assay can be used to investigate and optimise reaction conditions at 100 microL scale. Enzyme activity and stability as a function of various reaction parameters, including temperature, pH and co-solvent concentration, have been determined. Additionally, reactions have been scaled up from 100 microL to 25 mL under the optimal reaction conditions identified by the micro-scale process development activities. Excellent conversion (>99%) and enantioselectivity (>99% ee) were obtained.
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