荧光
显微镜
荧光显微镜
全内反射荧光显微镜
活力测定
辐照
生物物理学
薄层荧光显微镜
分辨率(逻辑)
化学
光学
材料科学
细胞
生物
生物化学
物理
核物理学
人工智能
计算机科学
作者
Michael Wagner,Petra Weber,Thomas Bruns,Wolfgang S. L. Strauß,Rainer Wittig,Herbert Schneckenburger
摘要
A test system for cell viability based on colony formation has been established and applied to high resolution fluorescence microscopy and single molecule detection. Living cells were irradiated either by epi-illumination or by total internal reflection (TIR) of a laser beam, and light doses where at least 90% of irradiated cells survived were determined. These light doses were in the range of a few J/cm2 up to about 200 J/cm2 depending on the wavelength of illumination as well as on the presence or absence of a fluorescent dye (e.g., the membrane marker laurdan). In general, cells were less sensitive to TIR than to epi-illumination. However, comparably high light doses needed for repetitive excitation of single molecules limit the application of super-resolution microscopy to living cells.
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