脚手架
椎间盘
生物医学工程
材料科学
肿胀 的
再生(生物学)
去细胞化
组织工程
解剖
细胞生物学
医学
复合材料
生物
作者
Zhen Li,Gernot Lang,Xu Chen,Hagit Sacks,Carmit Mantzur,Udi Tropp,Kerstin T. Mader,Thomas C. Smallwood,Chris Sammon,R. Geoff Richards,Mauro Alini,Sibylle Grad
出处
期刊:Biomaterials
[Elsevier]
日期:2016-01-21
卷期号:84: 196-209
被引量:52
标识
DOI:10.1016/j.biomaterials.2016.01.040
摘要
Nucleus pulposus (NP) replacement offers a minimally invasive alternative to spinal fusion or total disc replacement for the treatment of intervertebral disc (IVD) degeneration. This study aimed to develop a cytocompatible NP replacement material, which is feasible for non-invasive delivery and tunable design, and allows immediate mechanical restoration of the IVD. A bi-phasic polyurethane scaffold was fabricated consisting of a core material with rapid swelling property and a flexible electrospun envelope. The scaffold was assessed in a bovine whole IVD organ culture model under dynamic load for 14 days. Nucleotomy was achieved by incision through the endplate without damaging the annulus fibrosus. After implantation of the scaffold and in situ swelling, the dynamic compressive stiffness and disc height were restored immediately. The scaffold also showed favorable cytocompatibility for native disc cells. Implantation of the scaffold in a partially nucleotomized IVD down-regulated catabolic gene expression, increased proteoglycan and type II collagen intensity and decreased type I collagen intensity in remaining NP tissue, indicating potential to retard degeneration and preserve the IVD cell phenotype. The scaffold can be delivered in a minimally invasive manner, and the geometry of the scaffold post-hydration is tunable by adjusting the core material, which allows individualized design.
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