生物传感器
石墨烯
聚苯胺
沉积(地质)
材料科学
纳米技术
化学
聚合
生物
聚合物
沉积物
古生物学
复合材料
作者
Shenguang Ge,Kaiqing Wu,Yan Zhang,Mei Yan,Jinghua Yu
标识
DOI:10.1016/j.bios.2016.01.072
摘要
A multi-amplified paper-based electrochemical strategy using Pb(2+) dependent DNAzyme as the recognition unit for Pb(2+) detection was developed. In this work, flower-like reduced graphene (FrGO) was prepared utilizing flower-like ZnO as template, which was first one step grown on the gold nanoparticles modified paper working electrode (Au-PWE). After being treated with acid and then modified with Au, a novel sensor platform named Au/FrGO/Au-PWE with large specific surface area and good electrical conductivity was fabricated. The Mn2O3 nanoparticle-assembled hierarchical hollow spheres (H-Mn2O3) was served as nanocarrier to immobilize GOx, HRP and signal strand (S3), resulting to the formation of S3/H-Mn2O3/HRP/GOx bioconjugations. In the presence of Pb(2+), the DNAzyme (S1) was activated and the substrate strand (S2) was cleaved. After the incubation with S3/H-Mn2O3/HRP/GOx in 0.1M HAc-NaAc solution (pH 4.3) containing 30 mM aniline and 15 mM glucose, a readily measurable "turn-on" electrochemical signal could be measured. On the basis of the signal amplification strategy of Au/FrGO/Au-PWE sensing platform and S3/H-Mn2O3/HRP/GOx bioconjugations, the developed biosensor exhibited a good linear response toward over a wide range of concentration from 0.005 to 2000 nM.
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