细菌
酶
共价键
试剂
抗生素
化学
生物化学
荧光
致病菌
荧光团
抗生素耐药性
微生物学
抗药性
组合化学
生物
物理
物理化学
有机化学
量子力学
遗传学
作者
Wuyu Mao,Lingying Xia,Yaqun Wang,Hexin Xie
标识
DOI:10.1002/asia.201601344
摘要
Abstract The spread of antibiotic resistance in pathogenic bacteria has become one of the major concerns to public health. Improved monitoring of drug resistance is of high importance for infectious disease control. One of the major mechanisms for bacteria to overcome treatment of antibiotics is the production of β‐lactamases, which are enzymes that hydrolyze the β‐lactam ring of the antibiotic. In this study, we have developed a self‐immobilizing and fluorogenic probe for the detection of β‐lactamase activity. This fluorogenic reagent, upon activation by β‐lactamases, turns on a fluorescence signal and, more importantly, generates a covalent linkage to the target enzymes or the nearby proteins. The covalent labeling of enzymes was confirmed by SDS‐PAGE analysis and MALDI‐TOF mass spectrometry. The utility of this structurally simple probe was further confirmed by the fluorescent labeling of a range of β‐lactamase‐expressing bacteria.
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