Establishing of Pathological Evaluation Method for the Therapeutic Effect of Dexamethasone in a LPS-induced Mouse Acute Pneumonia Model

Dexamethasone was used as a positive drug to establish and validate a pathological evaluation method for a lipopolysaccharide(LPS) induced mouse acute pneumonia model,and develop a low-priced and easy preliminary pharmacodynamic evaluation model for the inflammatory and infectious diseases.The LPS-induced mouse acute pneumonia model was developed by the nasal inhalation of LPS.The pathology classification standard was set up via HE staining based on the lung injury severity against time points.Dexamethasone was adopted as a positive drug to validate the pathological evaluation method.After inhalation of LPS,the lungs were collected at different time points for pathological evaluation.A 0 – 5 level pathological classification standard of lung injury was established.This method was used to evaluate the therapeutic effect of the positive drug dexmethasone,and proved a significant difference between the LPS control and dexmethasone-treatment group.We established the pathological evaluation method for the therapeutic effect of dexamethasone in the LPS-induced mouse acute pneumonia model.The method can be used for the preliminary screening of anti-inflammatory drugs and their pharmacodynamic studies.