To investigate the chemical constituents from the whole plant of Loxocalyx urticifolius.The chemical constituents were isolated by chromatography and identified by MS, 1H NMR, 13C NMR and 2D-NMR methods.The structures were established as poliothrysoside, beta-sitosterol and stigmasterol, palmitic acid and tyrosine.This is the first time for the chemical constituents of L. urticifolius to be reported.