Chemical Constituents from Polyalthia oblique(I)

Objective: This study was carried out to study the chemical constituents from of Polyalthia oblique. Method: Compounds from 95% ethanolic extract of P. oblique were isolated by column chromatography on silica gel,Sephadex LH-20,together with recrystallization,and their structures were identified by their physical characteristics and spectral features. Result: Eight compounds were isolated from P. oblique,and identified as alkaloids isooncodine(1),pendulamine A(2),three steroids stigmasta-4-ene-3,6-dione(3),β-sitosterol(4),stigmasterol(5),and three other compounds including hydroquinone(6),vanillin(7),octadecadienoic acid(8). Conclusion: All compounds were isolated from P. oblique for the first time. Compound 3 showed inhibition activity against Escherichia coli,with minimal inhibitory concentrations(MIC) value of 5 mg·L- 1. Compound 1showed weak inhibition activities against SPC-A-1 and A-549 tumor cells,with IC50 values of 78. 6,49. 3 mg·L- 1.