化学
组氨酸
分子质量
黑色素
溶解度
蒸馏水
质谱法
葡聚糖
质子核磁共振
核磁共振波谱
分子
红外光谱学
色谱法
核化学
生物化学
立体化学
有机化学
氨基酸
酶
作者
Ziyang Ye,Ying Lu,Shuai Zong,Liuqing Yang,Farnaz Shaikh,Jinglei Li,Ming Ye
标识
DOI:10.1016/j.procbio.2018.09.007
摘要
Intracellular melanin (LIM) was extracted from Lachnum singerianum YM296 mycelium. LIM-a, LIM-b and LIM-c were resolved from LIM by Sephadex G-15 column chromatography, in which LIM-a was the main homogeneous component with a molecular weight of 530 Da. Based on the elemental analysis, mass spectrometry, infrared spectroscopy and NMR analysis, the molecular formula (C28H20N2O7S2) and possible structural formula of LIM-a were proposed. In order to increase its water solubility, non-water-soluble LIM-a was modified by histidine, lysine and arginine, and histidine-melanin (HLIM-a) had the highest water solubility, being 47.7 mg mL−1 in distilled water at room temperature. Infrared spectroscopy, mass spectroscopy and 1H NMR analysis of HLIM-a indicated that histidine-melanin was formed by conjugating LIM-a molecule with histidine molecule. In vivo test showed that both LIM-a and HLIM-a had significant anti-tumor activity, in which HLIM-a showed better efficacy. Immunohistochemistry analysis and cytokines detection suggested that LIM-a and HLIM-a may repress tumor growth through activating the immune response.
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