The Antimicrobial Peptide Nal-P-113 Exerts a Reparative Effect by Promoting Cell Proliferation, Migration, and Cell Cycle Progression

细胞生长 流式细胞术 伤口愈合 细胞周期 细胞迁移 细胞 细胞计数 分子生物学 化学 男科 生物 医学 免疫学 生物化学
作者
Liu Na-na,S B Guan,Hongyan Wang,Chen Li,Jya‐Wei Cheng,Huiyuan Yu,Li Lin,Yaping Pan
出处
期刊:BioMed Research International [Hindawi Publishing Corporation]
卷期号:2018: 1-10 被引量:11
标识
DOI:10.1155/2018/7349351
摘要

Objective . The primary purpose of this study was to evaluate the reparative efficacy of a novel antimicrobial peptide, Nal-P-113, in shortening the healing time of oral mucosal ulcers by promoting cell proliferation and migration and accelerating the cell cycle. Methods . Cell counting kit-8 (CCK-8) and wound-healing assays were used to evaluate the proliferation and migration of human immortalized oral epithelial cells (HIOECs). The cell cycle distribution of HIOECs was analyzed by flow cytometry. Additionally, the RNA levels of EGF, FGF-2, and TGF- β 1 of HIOECs were assessed by real-time PCR. Rats were divided into three groups randomly: (a) blank control group; (b) 20 μ g/mL Nal-P-113; and (c) 10 ng/mL rhEGF. An oral mucosal ulcer was induced in every rat by the application of 30% acetic acid. An immunohistochemical assay was used to assess the expression of EGF, FGF-2, and TGF- β 1 in the rat oral mucosa. Results . In the CCK-8 assay, the optical density values in the Nal-P-113 and rhEGF groups were found to be significantly higher than that in the blank control group. In addition, the scratch areas in the Nal-P-113 and rhEGF groups were found to be significantly smaller ( P <0.05). Cell cycle analysis showed that Nal-P-113 accelerated the entry of HIOECs into the S phase and expedited their cell cycles. The RT-PCR results suggested that Nal-P-113 upregulated the RNA levels of EGF and FGF-2 but downregulated that of TGF- β 1 at 24 h and 48 h. Lastly, the immunohistochemical assay verified that Nal-P-113 changed the expression of the above cytokines in rat mucosal ulcers. Conclusion . Nal-P-113 promoted the repair of oral mucosal ulcers by increasing the EGF and FGF-2 expression and decreasing that of TGF- β 1 in HIOECs, accelerating their proliferation and cell cycle progression. The application of Nal-P-113 might serve as an effective therapeutic approach for recurrent aphthous stomatitis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
死去的温柔5完成签到,获得积分10
刚刚
传奇3应助wjx采纳,获得10
1秒前
在水一方应助wjx采纳,获得10
1秒前
1秒前
酷波er应助wjx采纳,获得10
1秒前
在水一方应助wjx采纳,获得10
1秒前
华仔应助wjx采纳,获得30
1秒前
小二郎应助wjx采纳,获得10
1秒前
星辰大海应助wjx采纳,获得10
1秒前
赘婿应助wjx采纳,获得10
1秒前
Jasper应助wjx采纳,获得30
1秒前
充电宝应助wjx采纳,获得10
1秒前
tender完成签到,获得积分10
3秒前
3秒前
tan发布了新的文献求助10
4秒前
usora发布了新的文献求助10
5秒前
至幸发布了新的文献求助10
6秒前
supertkeb发布了新的文献求助30
7秒前
zhou应助wjx采纳,获得10
9秒前
科目三应助wjx采纳,获得10
9秒前
小桑桑发布了新的文献求助10
9秒前
JW关闭了JW文献求助
10秒前
12秒前
明亮的寒香关注了科研通微信公众号
12秒前
迅速仰完成签到 ,获得积分20
13秒前
量子星尘发布了新的文献求助10
14秒前
搞怪的紫雪完成签到,获得积分10
18秒前
FYZ完成签到,获得积分10
19秒前
默默的灵寒完成签到,获得积分20
20秒前
明理宛秋完成签到 ,获得积分10
24秒前
25秒前
123456发布了新的文献求助10
27秒前
工藤新一发布了新的文献求助10
29秒前
充电宝应助LL采纳,获得10
29秒前
30秒前
阳光的虔纹完成签到 ,获得积分10
30秒前
FYZ发布了新的文献求助10
32秒前
33秒前
34秒前
35秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3975426
求助须知:如何正确求助?哪些是违规求助? 3519848
关于积分的说明 11199831
捐赠科研通 3256122
什么是DOI,文献DOI怎么找? 1798124
邀请新用户注册赠送积分活动 877386
科研通“疑难数据库(出版商)”最低求助积分说明 806305