ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS OF THE METHANOLIC EXTRACTS OF LEAVES OF ACONITUM HETROPHYLLUM USING GAS CHROMATOGRAPHY-MASS SPECTROSCOPY (GC-MS) TECHNIQUE

Aconitum heterophyllum is herbal constituent of alpine pastures of Central Himalaya, has been used as a traditional medicine.The present study was conducted to check the GC-MS analysis of the Aconitum heterophyllum leaves methanolic extracts.The GC-MS analysis of the methanolic extract showed the presence of 60 compounds from A. hetrophyllum by comparison of their retention indices and mass spectra fragmentation patterns with those stored on the GC-MS computer library.The major compound identified were 2-Butenedioic acid (2E)-(10.99%),Cholestanol, 2-fromyl-3-benzyl-(10.58%),Phytol isomer (10.06%) with retention time 28.45, 46.35 and 26.98 minute respectively.The minor compound identified were 2,5-Dihydroxy-2,5-Dimethyl-3-Hexyne (0.19%), Hexanoic acid, 2-Methyl-(0.17%),Razoxane (0.14%) with retention time 18.33, 18.07 and17.56minute respectively.The study summarizes the information concerning the phytochemical constituents present in methanolic leaves extracts.These constituents may be responsible for pharmacological activities.