Acid‐treated Graphitic Carbon Nitride Nanosheets as Fluorescence Probe for Detection of Hemin

Abstract An acid‐treated, oxidized graphitic carbon nitride nanosheets (g‐C 3 N 4 NSs) was successfully synthesized and applied as a fluorescence probe for the detection of hemin based on the inner filter effect (IFE). The results indicated that the fluorescence quenching of g‐C 3 N 4 NSs caused by hemin was attributed to the overlap between absorption spectrum of hemin and emission spectrum of g‐C 3 N 4 NSs. It is confirmed that the g‐C 3 N 4 NSs exhibited a highly sensitive and selective for detection of hemin. Under the optical condition, the fluorescence changing of g‐C 3 N 4 NSs is related to the concentration of hemin ranging from 0.5 – 25 μM with a detection limit of 0.15 μM. Moreover, the fluorescence probe has been successfully applied to detect hemin in human blood. Overall, a novel and efficient fluorescence‐based method was developed supported by g‐C 3 N 4 NSs, which is a promising material for application in sensing and cell imaging.